Betti Worth, 31, a sports coach and gym owner from Bolton will be appearing on the show in the ultimate test of speed and strength.
Betti is the owner of ‘The Farm with Betti’ where she works as a fitness trainer, and she was previously a player for Chorley Women FC.
Speaking about making it onto the show Betti said: “I made it.
“This doesn’t seem real. I am so thankful for my friends and family.
“Everyone has talked about Gladiators with me for 9+ months. It started off as an unrealistic dream and here I am!
“I am Betti from Bolton and I’m going to be on BBC1 this Saturday night on Gladiators.
“I just want to shout it out loud to everyone I see always!
“I don’t have the words to explain how proud, thankful and happy I am. In fact, I’m tearing up.
“I have the best family and friends. Thanks for the crazy amount of support!”
The show will air on BBC1 at 5.50pm hosted by father and son duo Bradley and Barney Walsh and will be the final round of heats before the quarter finals.
It will also be available to watch on BBC iplayer.
Gladiators returned to screens last month as a BBC revival of the 1990s action game show.
The series launched to an average audience of 8.37 million for its first episode on BBC One on January 13.
Speaking on BBC Breakfast ahead of the series, one of the new Gladiators, known as Dynamite, confirmed the athletes would be paying compliments to contenders for the effort they put in.
She said: “As natural sports people we want to show all those younger kids that you can be competitive and it is okay to want to win, but we also want to show that if you lose, that’s also okay as well.
“There’s no point putting contenders down and making them feel bad.”
The 20-year-old full-time elite CrossFit athlete, whose real name is Emily Steel, revealed she is “quite nice” on the show but that villainous-style characters would return.